Frequently Asked Questions

General Testing Questions

Getting Started Testing

  • Yes! TUMI Genomics sample collection process renders the plant material “unusable and unrecognizable” and is legally defined as agricultural waste.

    The only plant tissue accepted in our testing laboratory is mature stalk. As indicated by the CSA, 21 U.S.C. § 802 (16) below, mature stalk is exempt from the definition of marijuana: "The term 'marihuana' means all parts of the plant Cannabis sativa L., whether growing or not; the seeds thereof; the resin extracted from any part of such plant; and every compound, manufacture, salt, derivative, mixture, or preparation of such plant, its seeds or resin. Such term does not include the mature stalks of such plant, fiber produced from such stalks, oil or cake made from the seeds of such plant, any other compound, manufacture, salt, derivative, mixture, or preparation of such mature stalks (except the resin extracted therefrom), fiber, oil, or cake, or the sterilized seed of such plant which is incapable of germination.”

    In addition, our sample collection process involves grinding of stalk tissue with greater than 50% glass and plastic, rendering the material unusable and unrecognizable according to to waste disposal rules in Colorado and other states (see section 3-230, E1 - https://www.sos.state.co.us/CCR/GenerateRulePdf.do?ruleVersionId=9900). The material sent to TUMI Genomics is legally defined as agricultural waste. The sample is also mixed on-site with a strong detergent which irreversibly binds all oils in the sample rendering them unusable.

  • TUMI Genomics can accept samples from anywhere in the world. We are already actively testing Hop Latent Viroid and other cannabis pathogens for growers in Canada, UK, France, The Netherlands, Spain, Italy, Austria, Switzerland, India, Soth Africa, Peru, Armenia, etc. Fill out our “contact us” form if you would like more information about international testing.

  • Each plant requires a single test. We suggest that growers collect 3-4 small petiole samples and 3-4 small upper root hairs and put all the tissue in the same tube. Careful studies on this sample collection method show that there is no decrease in sensitivity on our test when multiple pieces of tissue are processed as a pool in a single collection tube. This allows a dramatic increase in accuracy for our tests.

  • We recommend that you test your mother plants, which are the most important plants in your process, at least every six weeks for economically damaging pathogens. Screening a subset of clones via pooled testing is also important, especially if you are suffering, or have suffered, from an outbreak. Regular, consistent testing is critical to reduce economic exposure from pathogens and to protect your investment.

  • What pathogens to test for depends on the type of cultivation facility you have (indoor, greenhouse, living soil, hydroponics, etc) and what kinds of symptoms you are seeing in your plants. The two most economically damaging pathogens in cannabis are hop latent viroid and fusarium, so we recommend screening for these two pathogens frequently; every six weeks is ideal. If you would like more information, see our testing panels here.

  • Sample tubes are shipped out within 24 hours of ordering. During checkout, you can select from various shipping speeds depending on how quickly you need the tests. Normal ground shipping in the continental United States generally takes 3-4 days.

  • Sample tubes last up to three months at room temperature and up to 1 year in the refrigerator. You do not have to send back all the collection tubes at once, but can send them as you test your plants.

  • While the sample collection process is identical, we request that customers submit individual samples for different pathogens. This allows the lab to process each sample in a way that is ideal for testing of that specific pathogen.

  • When ordering online just follow the instructions to pay with a credit card or with the available digital payment platforms on the ecommerce site.

  • If you sign up for a testing program with us, you can have access to a discounted price. Contact us to set up a testing program for your grow.

Collecting Samples

  • TUMI Genomics has conducted detailed experiments on how to take samples for HLVd testing in collaboration with our customers. We recommend cutting 4 to 5 small petiole sections and 2-3 top root sections. The size of the sections are small (2-3 mm in length). The idea is to take several small sections instead of few big sections. For taking the root section the best way to do it is to gently rub your finger on top of medium close to main stem until small hair-like roots appear. Pull one of these roots with your hand using gloves and before cutting small section to put into tube, remove as much dirt/medium as possible. Leaf tissue is not very predictive of infection and can reduce the sensitivity of the test due to a high concentration of inhibitors.

    For fungal pathogens root tissue, especially from symptomatic areas, is the most accurate way to detect the pathogen. Foliage tissue can also be included if a systemic infection is suspected.

  • For an optimal sample, we request customers collect 3-4 small petiole samples from around the middle ⅔ of the plant and 3-4 top root samples near the stem. Each sample should be roughly half the length of your pinky fingernail. The goal when collecting samples isn’t to submit big pieces of tissue, but to submit many small pieces from around the plant.

  • An ideal sample includes 3-4 small pieces of petiole and 3-4 small pieces of upper root. All the tissue should be added to the same tube, one tube per plant.

  • Soil and other inert materials do not interfere with the assay. We do request that customers scrape off excess soil or growing medium from the root tissue with a paper towel or with their fingers prior to adding to the sample tube. If you have recently sprayed with sulfur please let us know.

  • Each collection kit comes with an extra set of stickers that match the numbers on each sample tube. Simply place the sticker with the same number as the sample to the pot of plant that samples was taken from.

  • Yes, we can test your growing medium for fungal and fungal-like pathogens. Contact us if you would like to discuss this type of testing.

  • Yes, TUMI genomics can also test seeds. The process is very similar to testing plant tissue. Contact us if you would like to submit seed samples.

  • No, a non-root test can still be accurate. The success rate of HLVd detection in older foliage is 75%. If you submitted 3-4 petiole samples from a positive plant, the chances of missing an infection are <1%. However, the levels of Hop Latent Viroid in foliage tissue is lower, so you are more likely to get a low-level/inconclusive result.

Shipping Samples

  • Ice packs are not necessary when shipping samples back to our lab. The stabilization solution included in the tube actually is more effective at higher temperatures, so sending the samples cold is not recommended.

  • While we encourage customers to send samples back to us as soon as possible after collection, samples are stable at room temperature for up to a week.

  • While refrigeration is generally not necessary if you will be keeping the samples more than a few days before sending them, refrigeration can help prolong the freshness of the tissue.

  • You can use any carrier (USPS, UPS, Fedex, DHL) to send samples back for testing. For international orders we recommend using UPS.

  • Please send your samples to:

    320 East Vine Drive, Suite 129,

    Fort Collins, CO

    80524

The Testing Process

  • Results are guaranteed to be delivered no later than 48 hours after arriving in the lab. Generally you will receive your results within 36 hours.

  • Your results will be sent to the email address you provided when you registered.

  • Each report contains a summary of the results, infection status for each sample, and the CT (cycle threshold) for each target which helps to estimate the amount of pathogen in each sample. Reports also include amplification curves from the PCR for each sample.

  • All of the tests run at TUMI Genomics lab are PCR tests. We perform TaqMan PCR, which has increased accuracy and sensitivity over standard PCR. All tests include an internal control in each sample as well as external controls that monitor assay performance. Our tests are extensively validated to confirm sensitivity, specificity, and accuracy.

  • TUMI Genomics assays are validated by the same standards set by the USDA and are optimized to dramatically reduce false results. We run a specialized type of PCR called TaqMan PCR, which is more sensitive and accurate than normal PCR. Each test also includes an internal control that detects cannabis RNA, which allows us to determine the quality and integrity of your sample. We have conducted extensive validation on all our assays and you can find that data here. Of course, it is important to remember that no diagnostic test, regardless of design is 100% correct, 100% of the time.

  • Each test includes an internal control that amplifies an RNA/DNA in all cannabis plants regardless of infection, so we can determine if your sample is of sufficient quantity and integrity to give an accurate result.

  • We guarantee the success of your samples. If your samples fail for any reason, we will retest a new sample at no charge.

  • If you have any questions about your results you can contact us by email, phone or instant message on our website. We are always happy to discuss results in more detail.

  • All results obtained from TUMI Genomics are strictly confidential. Your data is stored in a secure database that is not accessible to anyone but the team at TUMI Genomics and only for the purposes of processing your results. We never share individual test results with any entity other than directly with the customer who shipped us the samples. Any further analysis of data to help us improve our testing process is anonymized and aggregated so your personal data is completely protected.

I Have Results, Now What?

  • If your plants test positive it is likely that you have a pathogen outbreak in your facility. The best course of action is to identify which plants are infected and remove them promptly. Diseased plants should be disposed of in a closed container and not composted. We can help you build a testing schedule specifically tailored to your results and facility size that will help you to quickly get the outbreak under control.

  • If your plants test negative, congratulations! However, it is important to continue screening your plant regularly. The best way to prevent damage from a future outbreak is to catch infections quickly before they spread through your facility. Similar to car insurance, you don’t cancel insurance if you don’t crash. Continually testing keeps you protected.

  • A low-level positive result means that the PCR test detected the pathogen in the sample, but the level was very low. This result could indicate an early infection. However, because PCR is so sensitive, very low levels can also be caused by cross-contamination during sample collection. If a low-level result is detected for a critical variety, we suggest you isolate that plant and test again in a few weeks to confirm. Results that fall into the category are indicated in blue on your report.

  • A failed sample means that the internal control, which looks for a part of the plant genome (a plant RNA), failed to amplify. Without robust amplification of the internal control we cannot be certain the integrity and concentration of the sample was sufficient to have been able to detect the infection. We rerun failed samples twice and guarantee that if your sample is not successful, we will run a new sample at no cost to you.

  • Because viroids can exist at variable levels throughout a plant, it is possible that two samples taken from different places around the same plant have drastically different amounts of viroid, such that one sample tests positive, while the other tests negative. We recommend taking 3-4 samples from older growth petioles and 3-4 samples from surface root and including them all in the same tube. Including multiple small pieces of tissue from around the plant allows the test to determine a complete picture of that plant's infection status.

Hop Latent Viroid Questions

Hop Latent Viroid - General

  • Hop latent viroid (HLVd) is a miniature molecular parasite that is pathogenic (i.e. causes disease) in cannabis and hops plants. HLVd is composed of a single circular, highly structured RNA that replicates by hijacking the molecular machine that normally interprets the plant’s DNA (RNA polymerase II) and forces that machine to copy the viroid instead.

  • There are many different types of pathogens that cause disease in cannabis plants including fungi, oomycetes (fungus-like), viruses, and bacteria. While these pathogens have distinct biology, one feature they share is that the genetic information of these pathogens is protected by outside coat. This coat, composed of fat, carbohydrates, proteins or all three, is essential for the pathogen’s survival. Viroids, on the other hand, do not have an outside coat. They are literally an unprotected, free-floating piece of genetic material. What this means is that disinfectants that work by breaking up fats or proteins (i.e. soap, alcohol, etc) do not work to destroy viroids.

  • Hop latent viroid was first identified in Hops plants in 1987. Due to the genetic similarities between hops and cannabis at some point, no one knows when this disease cross-species jumped into cannabis. Given the wide geographical distribution of hop latent viroid in cannabis, this pathogen has likely been spreading throughout the industry for a long time.

  • Hop latent viroid is prevalent in hops plants worldwide. However, there is no published data describing the geographical distribution of hop latent viroid in cannabis. At TUMI Genomics we have conducted more than 18,000 PCR tests and have identified HLVd in almost every state in the USA, including Alaska and Hawaii, and impacts 73% of the cultivation facilities. We have also characterized infections in Canada and the United Kingdom. Based on this data and the wide geographical spread in hops plants, hop latent viroid is very likely a global cannabis pandemic.

  • Hop latent viroid has a fairly narrow host range. The only known natural hosts are hops, cannabis and stinging nettle. Because viroids mutate quickly, it is possible that hop latent viroid could adapt to infect other crops in the future.

  • Hop latent viroid is having devastating impacts on the cannabis industry. Estimates put the industry loss due to this pathogen upwards of 4 billion dollars per year. Based on our testing data, hop latent viroid is present across the United States and affects 73% of all growers.

Transmission of Hop Latent Viroid

  • The major mode of transmission for hop latent viroid is mechanical. Transmission occurs when sap from an infected plant gains access to a wound in a non-infected plant. One of the most efficient ways this can occur is by trimming plants with improperly sterilized cutting tools. Other possible modes of transmission include, seed transmission, water transmission, and insect transmission.

  • While there is no published evidence that insect transmission of Hop latent viroid occurs, numerous other viroids can be transmitted via insects, so insect transmission of HLVd is likely.

  • Yes, hop latent viroid is transmitted through seeds. Current published evidence in hops plants indicates this percent transmission is about 8%. The exact percent transmission via seeds in cannabis is not known.

  • Hop latent viroid can remain infectious on cotton and leather for 24 hours or more and about 3 hours on human skin. We strongly recommend individuals touching plants wear personal protective clothing and gloves. While trimming, gloves should be sterilized between each plant using 10% bleach. The bleach can be placed in a spray bottle and used on gloved hands similar to hand sanitizer. There is no need to rinse the bleach off the gloves before moving to the next plant.

  • The root system of plants contains a high level of viroid. If planting seeds in soil that still contains root mass from an infected plant, there could be a possibility that the infection will be transmitted to the next plant. Reusing soil from sick plants, whether they were sick with hop latent viroid or something else, is not recommended. Coco can be sterilized with bleach between uses to remove viroid and other pathogen contamination.

  • In order for hop latent viroid to spread infected sap must gain contact with a wound in another plant. If the rubbing between two plants resulted in damage or micro-tears, it is possible that the viroid could spread. However, two plants simply sharing the same physical space does not guarantee transmission. Shared water could transmit viroids between sick and healthy plants, so care should be taken to avoid shared water between plants of unknown infection status.

Symptoms and Treatment of Hop Latent Viroid

  • Hop latent viroid can have variable effects on infected plants. Plants can be stunted, grow slowly, display horizontal branching and brittle stems, leaf discoloration, and dramatically reduced flower size. Plants infected with hop latent viroid often show more severe symptoms when stressed. However, plants can also harbor asymptomatic infections that are still infectious, so the only way to be sure plants are free from hop latent viroid is by testing.

  • While there are a lot of theories as to the cause of symptoms due to viroids, the theory that has the most robust evidence is that viroids cause an auto-immune type response in the plant. The plant’s version of the secondary immune response (called RNA-interference) attempts to respond to the presence of the viroid, but this response backfires and the plant ends up attacking itself instead. If a critical component of the immune system (RNA-interference pathway) is removed, plants do not develop symptoms from viroid infections and if the immune system is artificially activated (without a viroid being present), plants mimic viroid-like symptoms.

  • The amount of time it takes for a plant to display symptoms depends on a lot of variables including plant age, plant size, the specific variety, and plant stress. Some plants don’t show severe symptoms until flowering. How long it takes an infected plant to build up enough viroid concentration to test positive, also depends a lot on plant size. For a small plant, 3 weeks is likely sufficient. However, in a very large mother plant, it can take months for the viroid to establish a systematic infection.

  • Yes, hop latent viroid can exist at variable levels around the plant. The reason for this phenomenon is due to the way viroids travel through the plant. Viroids are dependent on the movement of sap through the vascular system to gain access to the entire plant. Viroids will accumulate in areas sap is moving into (such as active growth), but to a lesser extent in areas where sap is moving away (such as old growth). This movement pattern occurs at the time of infection, meaning new growth when the plant was infected is likely older growth on the same plant several months later. Because the timing of infection is likely unknown, it is difficult to predict which branches contain high levels of viroid and which do not.

  • There is no topical treatment for viroids. You cannot cure a plant using a spray or by dipping a clone in a solution. Meristem tissue culture can remove viroids from a critical variety, but the process is time consuming and can be costly. The best way to treat hop latent viroid is to prevent it from entering and spreading in your facility through sanitation and regular testing.

Decontamination of Viroids

  • Viroids can remain infectious on most common surfaces for more than 24 hours, 8 days on contaminated tools, and up to 7 weeks in water.

  • The most effective way to destroy hop latent viroid on surfaces and tools is with household bleach diluted to 10% (1 part bleach in 9 parts water). A contact time of sixty seconds or more will inhibit transmission of viroids.

  • Viroid contamination on tools can be removed using 10% bleach with a contact time of one minute or more. We recommend trimming plants with multiple pairs of scissors in a jar of 10% bleach. When you move from one plant to another, drop the dirty scissors in the bleach solution and take a fresh pair from the bleach jar. Rotate to a pair of scissors that have been in the bleach for a minute or more when you move to the next plant. The bleach solution should be replaced if saturated with plant material.

  • The active ingredient in bleach that destroys viroid contamination is sodium hypochlorite. Confirm the concentration in the commercial bleach you purchase is >6% (most are between 5.25% to 8.5%). Many splash-less bleach solutions fall below this concentration so be sure to confirm the sodium hypochlorite concentration. Calcium hypochlorite has the same mechanism of action as sodium hypochlorite and is an acceptable substitute.

  • A 10% bleach solution is made by combining

    1 part concentrated bleach (>6% sodium hypochlorite)

    9 parts clean water

    The final concentration of the active ingredient will be >0.5%, which is broadly effective against viroids, including hop latent viroid.

  • Diluted bleach solutions should be replaced at least every two weeks and whenever they become cloudy or saturated with plant material. A 20% bleach solution can also be used (1 part bleach, 4 parts water) to extend the life of a dilute solution.

  • While alcohol can be effective to sterilize against many pathogens, it is NOT effective at removing viroid contamination. Alcohol actually stabilizes viroids, which increases the rate of transmission between plants. Bleach can leave a salt residue after drying and alcohol can be used to remove this residue. However, care should be taken to NOT mix liquid bleach and alcohol, as this makes a toxic gas (chloroform). Alcohol can also be used to remove plant matter before bleaching as long as a bleach step is included after.

  • Heat sterilization is not completely effective at deactivating viroids. Experiments have shown that a propane blow torch or extended heating in an oven does not guarantee viroid decontamination. Heat should not be the only method of viroid sterilization that is used, especially on contaminated cutting tools.

Testing for Hop Latent Viroid

  • The presence of hop latent viroid cannot be determined by symptoms alone. Even if you do not see any major symptoms, it is critical to test your mother plants to confirm no pathogens are present that could cause serious economic consequences at your facility.

  • Hop latent viroid must be detected by a molecular test, such as PCR. A molecular test (or a test that looks for the genome of the pathogen) is required because viroids do not contain any other component besides genetic material. Due to the initially asymptomatic nature of Hop Latent Viroid and how quickly the disease can spread, we recommend screening your mother plants every 4-6 weeks.

  • Viroid load estimates the amount of viroid in your sample. This number can help you understand the severity of a potential infection. However, the significance of a low-level viroid infection is not clear. There is no cure for viroids in any agriculture crop besides tissue culture nor evidence that a sick plant can recover or “heal” itself. The normal mechanism that plants use to combat viral infections (RNA-interference) is not efficient on viroids. Because of this, the safest route is to treat low-level infections as potential sources of spread and isolate or remove those plants.